{"id":1148,"date":"2022-04-25T16:46:37","date_gmt":"2022-04-25T21:46:37","guid":{"rendered":"https:\/\/research.knight.domains\/urf\/?p=1148"},"modified":"2022-04-28T13:35:07","modified_gmt":"2022-04-28T18:35:07","slug":"functional-analysis-of-protonephridia-genes-identified-by-drop-seq-2","status":"publish","type":"post","link":"https:\/\/research.knight.domains\/urf\/2022-presentations\/functional-analysis-of-protonephridia-genes-identified-by-drop-seq-2\/","title":{"rendered":"Functional Analysis of Protonephridia Genes Identified by Drop-Seq"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>Andrew Gaetano<\/strong> Biology, Biomedical<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Ryan King <\/strong>Associate Professor of Biology<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Presentation Time:<\/strong> April 28, 3:30pm &#8211; 4pm<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"720\" height=\"720\" src=\"https:\/\/research.knight.domains\/urf\/wp-content\/uploads\/2022\/04\/Slide1.jpeg\" alt=\"\" class=\"wp-image-1320\" srcset=\"https:\/\/research.knight.domains\/urf\/wp-content\/uploads\/2022\/04\/Slide1.jpeg 720w, https:\/\/research.knight.domains\/urf\/wp-content\/uploads\/2022\/04\/Slide1-300x300.jpeg 300w, https:\/\/research.knight.domains\/urf\/wp-content\/uploads\/2022\/04\/Slide1-150x150.jpeg 150w, https:\/\/research.knight.domains\/urf\/wp-content\/uploads\/2022\/04\/Slide1-400x400.jpeg 400w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><a href=\"https:\/\/research.knight.domains\/urf\/wp-content\/uploads\/2022\/04\/Gaetano-Andrew-2022-Spring-URF-Poster-FINAL-DRAFT.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">View Poster<\/a><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Abstract<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Schmidtea mediterranea, the freshwater planarian, possesses protonephridia, which are an organized network of branched tubules that function in waste filtration and water regulation. On a cellular and molecular level, these structures show considerable homology to the human kidney making planarians a useful model system for exploring human excretory biology and disease (Scimone, 2011 and Vu, 2015). Additionally, the planarian genome contains many flatworm-specific protonephridia genes essential for survival, making them potential drug targets for parasitic worm infections of humans. We set out to validate the expression of protonephridia genes identified by Drop-seq, a single-cell RNA sequencing technique that identified the transcriptomes for every cell type in S. mediterranea (Fincher et al., 2018). To accomplish this, we mapped gene expression using colorimetric and fluorescence in situ hybridization and characterized gene functionality via RNA interference screening (RNAi).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Andrew Gaetano Biology, Biomedical Ryan King Associate Professor of Biology Presentation Time: April 28, 3:30pm &#8211; 4pm View Poster Abstract Schmidtea mediterranea, the freshwater planarian, possesses protonephridia, which are an organized network of branched tubules that function in waste filtration and water regulation. On a cellular and molecular level, these structures show considerable homology to [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":1320,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14],"tags":[20],"class_list":["post-1148","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-2022-presentations","tag-poster","has-thumbnail"],"_links":{"self":[{"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/posts\/1148","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/comments?post=1148"}],"version-history":[{"count":5,"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/posts\/1148\/revisions"}],"predecessor-version":[{"id":1336,"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/posts\/1148\/revisions\/1336"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/media\/1320"}],"wp:attachment":[{"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/media?parent=1148"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/categories?post=1148"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/research.knight.domains\/urf\/wp-json\/wp\/v2\/tags?post=1148"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}